LUCRARI ŞTINTIFICE MEDICINA VETERINARĂ.
TIMIŞOARA. VOL. XL, 120 - 123. 2007
Normal blood glucose in White
Wistar rat and its changes following anesthesia
Brăslaşu Elena Daniela, Brădăţan Cătălina, Cornilă
Mihai,
Săvulescu Ilinca*, Cojmăleaţă Roxana*,
Brăslaşu M.C.
Veterinary Faculty Bucharest
* U.M.F.
Carol Davila, University
Hospital Bucharest
Summary
The studies have been made in
110 White Wistar Rats, both male and female, the animals being in wakeful
state. The sampling have been made from coccygeal vertebra and measurement of
the blood glucose were made with Accu-Chek Go (Roche) glucometer. The blood
glucose values were 117,06±1,96 mg/dl. It is showed that the anesthesia produced hyperglycemia.
White Wistar Rats is an animal frequently used in
different experiments from different
fields of medical pathology. One of these is diabetes mellitus that’s
way it was necessary to study the way regarding the determination of blood
glucose, the normal values of blood
glucose and the changes produced by anesthesia.
Materials and method
There were examined 110 White
Wistar Rats, bolt male and female. The animals were clinical healthy with 150 -220
gr. body weight. The blood glucose has
been determined through two methods: 1. with Accu-Chek Go glucometer; 2. with
reflotron.
The blood glucose has been
measured only wakeful state animals and on animals that were anesthesiated:
Xylazina - 5 mg/kg and Ketamina – 35 mg/kg.
The blood glucose values were
statistically interpreted by calculating: the average, the variance, the media
error, standard deviation and the coefficient of variability.
Results and discussion
A. Technical parts
The blood has been collected
from the coccygeal vertebra because there is a blood trace and that’s why
Accu-Chek Go glucometer was used. The blood glucose determinations were made in
the morning before meals.
The technique was as follow
(photo 1):
1. setting the patient in
standing position;
2. puncture the coccygeal
vertebra with a thick needle;
3. the obtaining blood drops was
“caught’ on glucometer, and
4. reading the values of blood
glucose in White rats with glucometer.
Photo 1
Taster determination of blood
glucose in White Wistar rats with glucometer
For determination the blood
glucose on reflotron, the blood has been obtained when the slaughter was done.
The differences between the
determinations made on glucometer and reflotron were upper in glucometer
measurements with 5-8 mg/dl.
B. Blood glucose values
The body weight of the rats
were 219,81±6, 49 gr. (n = 110). The blood glucose values are in table 1.
Table 1
Blood glucose values in White
Wistar rats
- method Accu-Chek Go,
glucometer –
Blood glucose mg/dl
|
|
Statistical indices
|
Values
|
n.
|
107
|
Average
|
117,06
|
Variance
|
412,74
|
Standard deviation
|
20,31
|
The media error
|
1,96
|
The coefficient of
variability %
|
17,35
|
From this table we observed
that the blood glucose values is
117,06±1,96 mg/dl. Bibliographic literature shows values of blood glucose as 50
– 125 mg/dl (Johnson-Delaney, C.,
1996) and 85 –
132 mg/dl (Kohn D.F., Clifford
C.B., 2002).
In this way the recent values
obtained are in the limits proposed by other authors.
The table also shows that the
value 17,35% of variability coefficient represents a media variability
coefficient (10 – 20%)
C. The influence of
anaesthesia in blood glucose
Regarding that the anesthesia
is an usual method of chemical setting, the blood glucose was initially measure after xylayine
and than after ketamine.
In this way, it’s showed that
both xylayine and ketamine give important increased of glycemia (table 2) so
that the measurements of blood glucose were done only in wakeful state.
In the clinic, in dog, there
were paradoxical situations when in diabetes mellitus, the anesthesia gave
hypoglycemia.
The same situation was seen to
a white rat, a female one of 280 gr. body weight. The female rat measured a
blood glucose of 135 mg/dl before the anesthesia and after the anesthesia was
done with ketamine the values of blood glucose were 90 mg/dl.
Table 2
Examples regarding, the
increase of blood glucose (mg/dl)
following the anesthesia
(xylayine-ketamine) in White Wistar rats
Nr.
|
Blood glucose (mg/dl)
|
||
Initial
wakefulness
|
After
anesthesia
|
%
|
|
1.
|
155
|
203
|
+ 30,9
|
2.
|
135
|
286
|
+ 111,8
|
3.
|
108
|
189
|
+ 75
|
4.
|
134
|
241
|
+ 79,8
|
5.
|
126
|
256
|
+ 103,1
|
6.
|
145
|
245
|
+ 68,9
|
7.
|
159
|
281
|
+ 76,7
|
Conclusions
1.
Blood glucose values in White Wistar rats is done in wakeful state from
coccygeal vertebra. The measure og blood
glucose can be done using glucometer – ex. Accu-Chek Go (Roche), tha
values obtained being upper than were done on reflotron.
2.
The values og blood glucose in White Wistar rats are 117,06±1,96 mg/dl.
3.
The anesthesia gives upper values of blood glucose (hyperglycemia).
That’s could appear errors in diagnostic (diabetes mellitus, pancreatic
disease).
References
1.
Brăslaşu
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pacienţii cu diabet zaharat. Contract cercetare CEEX, 55/2005. Contract VIASAN.
Director de contract. Prof. I. Bruckner.
2.
An D.,
Rodrigues B., 2006 - Role of changes in
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4,H1489-506.
3.
Cheţa D.,
2002 – New Insights into experimental diabetes. Editura Academiei Române,
Bucureşti.
4.
Johnson-Delaney, C., 1996 - Exotic Animal Companion Medicine Handbook
for Veterinarians, Zoological Education Network.
5.
Kohn
D.F., Clifford C.B., 2002 - Biology and
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Animal Medicine, 2nd ed. New York: Academic Press, 121-167.
